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Harley Quinn (Harleen Frances Quinzel) is a fictional supervillain appearing in American comic books published by DC Comics. The character was created by Paul Dini and Bruce Timm, and first appeared in Batman: The Animated Series in September 1992. She later appeared in DC Comics' Batman comic books, with her first comic book appearance in The Batman Adventures #12 (Sept. 1993).

Harley Quinn is the Joker's frequent accomplice and lover, whom she met while working as a psychiatrist at Arkham Asylum, where the Joker was a patient. Her name is a play on the name "Harlequin", a character which originated in the commedia dell'arte.